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Small-angle-X-ray scattering (SAXS)

With Small-Angle-X-ray-Scattering the size, shape and oligomeric state of biological macromolecules in solution can be determined. SAXS can provide detailed information within a scale of one to several hundred nanometers which represents the relevant range for macromolecular samples. Small-Angle-X-ray-Scattering allows to examine all samples in native aqueous solution without any marker or crystallization which can be of crucial advantage. A special possibility is the analysis of protein complexes and their stoichiometry as well as the spatial orientation of the individual subunits to each other. 

For biological macromolecules (e.g. proteins, DNA, sugars, amphiphiles) SAXS can resolve the structure in solution which also includes transition state structures like protein complexes or -aggregates, dynamic processes like ligand binding, conformational changes and reaction cycles, as well as interactions between proteins. Additionally flexible parts within a protein can be identified (e. g. intrinsically disordered proteins).

The CSS has regular access for SAXS beam time at the synchrotrons in Hamburg and Grenoble and offers the data collection as service for all members of the HHU and external users. 

Furthermore, the CSS is about to schedule in house SAXS experiments. This will allow to optimize macromolecular experiments performed at synchrotrons on one hand, on the other hand SAXS experiments will become accessible for small (anorganic or organic) molecules, colloids or cross-linked microgels.

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